More Positive or More Negative? Metagenomic Analysis Reveals Roles of Virome in Human Disease-Related Gut Microbiome

• DOI: 10.3389/fcimb.2022.846063
• Publication Date: 2022-04-12T13:55:42Z
• Authors: Mo Li; Chunhui Wang; Qian Guo; Congmin Xu; Zhongjie Xie; Jie Tan; Shufang Wu; Peihong Wang; Jinyuan Guo; Zhencheng Fang; Shiwei Zhu; Liping Duan; Xiaoqing Jiang; Huaiqiu Zhu
• Keywords: Human virome; Dysbiosis

Viruses are increasingly viewed as vital components of the human gut microbiota, while their roles in health and diseases remain incompletely understood. Here, we first sequenced analyzed 37 metagenomic 18 host metabolomic samples related to irritable bowel syndrome (IBS) found that some shifted viruses between IBS controls covaried with bacteria metabolites. Especially, phages infect beneficial lactic acid depleted hosts. We also retrieved public whole-genome datasets another four (type 2 diabetes, Crohn’s disease, colorectal cancer, liver cirrhosis), totaling 438 including IBS, performed uniform analysis diseases. By constructing disease-specific co-occurrence networks, actively interacting bacteria, negatively correlated possible dysbiosis-related inflammation-mediating increasing connectivity modules, contributing robustness networks. Functional enrichment showed interact through predation or expressing genes involved transporter secretion system, metabolic enzymes, etc . further built a viral database facilitate systematic functional classification explored functions on bacteria. Our analyses provided view virome disease-related microbial community suggested positive concerning health.