Assessment of IgG3 as a serological exposure marker for Plasmodium vivax in areas with moderate–high malaria transmission intensity

• DOI: 10.3389/fcimb.2022.950909
• Publication Date: 2022-08-09T05:55:48Z
• Authors: Yanie Tayipto; Jason Rosado; Dionicia Gamboa; Michael T. White; Benson Kiniboro; Julie Healer; D. Herbert Opi; James G. Beeson; Eizo Takashima; Takafumi Tsuboi; Matthias Harbers; Leanne Robinson; Ivo Mueller; Rhea J. Longley
• Keywords: Multiplex

A more sensitive surveillance tool is needed to identify Plasmodium vivax infections for treatment and accelerate malaria elimination efforts. To address this challenge, our laboratory has developed an eight-antigen panel that detects total IgG as serological markers of P. exposure within the prior 9 months. The value these been established use in areas with low transmission. In moderate–high transmission areas, there evidence long-lived than transmission, resulting poorer performance settings. Antibodies are shorter-lived may be better recent infection areas. Using a multiplex assay, antibody temporal kinetics IgG, IgG1, IgG3, IgM against 29 P . antigens were measured over 36 weeks following asymptomatic Papua New Guinean children ( n = 31), from area intensity. IgG3 declined faster background IgM. Based on kinetics, was then assessed classifying cohort Peruvian individuals 590; age 3–85 years) moderate responses individual antigens, highest months one Pv-fam-a proteins (PVX_125728) (AUC 0.764). Surprisingly, overall marker infection, classification RBP2b 1986-2653 (PVX_094255) 0.838). understand acquisition cohort, relevant epidemiological factors explored using regression model. levels positively associated increasing age, living (relatively) higher intensity, having three or PCR-detected blood-stage 13 Overall, we found did not have high accuracy detecting data suggesting due required acquire levels.