Recombinant actin-depolymerizing factor of the apicomplexan Neospora caninum (NcADF) is susceptible to oxidation

• DOI: 10.3389/fcimb.2022.952720
• Publication Date: 2022-12-19T11:37:12Z
• Authors: Luciana Baroni; Péricles Gama Abr...; Luiz Miguel Pereira; Markus Nagl; Ana Patricia Yatsuda
• Keywords: 0301 basic medicine; N-chlorotaurine; Neospora; Neospora caninum; actin-binding protein; Hydrogen Peroxide; actin-depolymerizing factor (ADF); Oxidants; Microbiology; QR1-502; Actins; 03 medical and health sciences; Cellular and Infection Microbiology; Destrin; Actin Depolymerizing Factors; Pregnancy; redox; Animals; Female; Cattle; Cysteine; Apicomplexa; Oxidation-Reduction

Neospora caninum is a member of Apicomplexa Phylum and the causative agent neosporosis, disease responsible for abortions in cattle. Apicomplexan parasites have limited set actin-binding proteins conducting regulation dynamics nonconventional actin. The parasite actin-based motility implicated invasion process host cell. Once no commercial strategy neosporosis control available, interference actin function may result novel drug targets. Actin-depolymerization factor (ADF) ADF/cofilin family, primarily known its severing depolymerization. ADF/cofilins are versatile modulated by different mechanisms, including reduction oxidation. In apicomplexan parasites, mechanisms involved modulation ADF barely explored effects oxidation protein unknown so far. this study, we used oxidants N-chlorotaurine (NCT) H2O2 to investigate susceptibility recombinant N. (NcADF) After exposing either NCT or H2O2, dimerization status cysteine residue were determined. Also, NcADF interaction with was assessed. treatment reversibly induced production dimers, indicating that disulfide bonds between residues formed. addition, exposure resulted more efficient compared H2O2. Finally, reduced ability altered polymerization. Altogether, our results clearly show sensitive redox conditions, cellular processes involving be