Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes

• DOI: 10.3389/fcimb.2023.1170763
• Publication Date: 2023-05-31T09:07:25Z
• Authors: Joachim Müller; Matías Preza; Marc Kaethner; Reto Rufener; Sophie Braga; Anne-Christine Uldry; Manfred Heller; Britta Lundström-...
• Keywords: Echinococcus multilocularis; Metacestode; Echinococcus

The larval stage of the cestode Echinococcus multilocularis is causative agent alveolar echinococcosis. To investigate biology these stages and to test novel compounds, metacestode cultures represent a suitable in vitro model system. These metacestodes are vesicles surrounded by an envelope formed vesicle tissue (VT), which laminated germinal layer, filled with fluid (VF). We analyzed proteome VF VT liquid chromatography tandem mass spectrometry (LC-MS/MS) identified total 2,954 parasite proteins. most abundant protein was expressed conserved encoded EmuJ_000412500, followed antigen B subunit AgB8/3a EmuJ_000381500 Endophilin B1 (protein p29). In VF, pattern different dominated AgB subunits. three other total, subunits detected represented 62.1% culture media (CM), 63 E. proteins were detected, made up 93.7% All (encoded EmuJ_000381100-700, corresponding AgB8/2, AgB8/1, AgB8/4, AgB8/3a, AgB8/3b, AgB8/3c) also found CM, except EmuJ_000381800 (AgB8/5) that very rare not CM. relative abundance CM same pattern. VT, only (AgB8/3a) EmuJ_000381200 (AgB8/1) among 20 see whether this specific from cultured metacestodes, we grown mouse model. Here, EmuJ_000381100-700 constituted proteins, namely, 81.9% protein, order as vitro. Immunofluorescence on showed co-localized calcareous corpuscles multilocularis. Using targeted proteomics HA-tagged EmuJ_000381100 (AgB8/2), could show uptake into occurs within hours.