Nipah virus attachment glycoprotein ectodomain delivered by type 5 adenovirus vector elicits broad immune response against NiV and HeV

• DOI: 10.3389/fcimb.2023.1180344
• Publication Date: 2023-07-27T15:36:06Z
• Authors: Xiaoyan Huang; Yaohui Li; Ruihua Li; Shaoyan Wang; Lu Yang; Shuyi Wang; Ying Yin; Xiaodong Zai; Jun Zhang; Junjie Xu
• Keywords: Immunogen; Hendra Virus; Ectodomain

Nipah virus (NiV) and Hendra (HeV) are newly emerging dangerous zoonotic pathogens of the Henipavirus genus Paramyxoviridae family. NiV HeV (HNVs) which transmitted by bats cause acute respiratory disease fatal encephalitis in humans. To date, as there is a lack antiviral drugs or effective therapies, development vaccines against those two viruses primary importance, immunogen design crucial to success vaccines. In this study, full-length protein (G), ectodomain (Ge) head domain (Gs) attachment glycoprotein were delivered replication-defective type 5 adenovirus vector (Ad5) respectively, recombinant Ad5-NiV vaccine candidates (Ad5-NiVG, Ad5-NiVGe Ad5-NiVGs) constructed their immunogenicity evaluated mice. The results showed that all stimulated specific humoral cellular immune responses efficiently rapidly both HeV, elicited strongest after single-dose immunization. Furthermore, potent conserved T-cell epitope DTLYFPAVGFL shared was identified may provide valid information on mechanism HNVs-specific immunity. summary, study demonstrates could be candidate HNVs inducing robust responses.