Diarrheal-associated gut dysbiosis in cancer and inflammatory bowel disease patients is exacerbated by Clostridioides difficile infection

• DOI: 10.3389/fcimb.2023.1190910
• Publication Date: 2023-07-27T15:40:35Z
• Authors: Maria Kulecka; Natalia Zeber-Lub...; Aneta Bałabas; Paweł Czarnowski; Katarzyna Bagińska; Maria Głowienka; Anna Kluska; Magdalena Piątkowska; Michalina Dąbrowska; Edyta Waker; Michał Mikula; Jerzy Ostrowski
• Keywords: Dysbiosis; Faecalibacterium prausnitzii

Introduction Low diversity gut dysbiosis can take different forms depending on the disease context. In this study, we used shotgun metagenomic sequencing and gas chromatography–mass spectrometry (GC-MS) to compared metabolomic profiles of Clostridioides (Clostridium) difficile diarrheal cancer inflammatory bowel (IBD) patients defined additive effect C. infection (CDI) intestinal dysbiosis. Results The study cohort consisted 138 case-mix patients, 43 IBD 45 healthy control individuals. Thirty-three were also infected with . group, three well-known enterotypes identified, while other groups presented an additional Escherichia -driven enterotype. Bacterial was significantly lower in all than controls, highest level bacterial species richness observed patients. Fifty-six had abundance levels that differentiated patient from group. Of these species, 52 4 ( Bacteroides fragilis , coli Klebsiella pneumoniae Ruminococcus gnavus ) under-represented over-represented, respectively, groups. relative abundances propionate butyrate fecal samples CDI samples. Isobutyrate, propanate, concentrations cancer, IBD, samples, respectively. Glycine valine amino acids over- represented Conclusion Our data indicate external internal factors drive comparable low While diarrheal-related may be a consequence systemic therapy, similar phenotype is cases moderate severe both cases, exacerbated by incidence CDI.