A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma

570 Phytoplasma [SDV]Life Sciences [q-bio] LRR proteins Membrane Proteins VmpA Microbiology 630 QR1-502 Insect Vectors protein-protein interaction Hemiptera Cellular and Infection Microbiology RNAi Euscelidius variegatus phytoplasma Animals Staphylococcal Protein A Adhesins, Bacterial Plant Diseases
DOI: 10.3389/fcimb.2023.1289100 Publication Date: 2023-11-06T16:23:47Z
ABSTRACT
Introduction The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells their insect vectors is partially mediated by variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order identify receptor for VmpA, we identified Euscelidius variegatus cell proteins interacting with recombinant VmpA-His 6 . Methods E. were mass spectrometry analysis VmpA- complexes formed upon in vitro interaction assays. To assess impact VmpA binding, reduced expression candidate genes on culture dsRNA-mediated RNAi. effect gene knockdown binding was measured capacity bind VmpA-coated fluorescent beads. Results and discussion There 13 possessing potential N-glycosylation sites predicted transmembrane domains selected. decrease unknown leucine-rich repeat (uk1_LRR) correlated decreased beads cells. uk1_LRR more expressed digestive tubes than salivary glands could be implicated early stages infection following phytoplasmas ingestion
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