A knockdown gene approach identifies an insect vector membrane protein with leucin-rich repeats as one of the receptors for the VmpA adhesin of flavescence dorée phytoplasma
570
Phytoplasma
[SDV]Life Sciences [q-bio]
LRR proteins
Membrane Proteins
VmpA
Microbiology
630
QR1-502
Insect Vectors
protein-protein interaction
Hemiptera
Cellular and Infection Microbiology
RNAi
Euscelidius variegatus
phytoplasma
Animals
Staphylococcal Protein A
Adhesins, Bacterial
Plant Diseases
DOI:
10.3389/fcimb.2023.1289100
Publication Date:
2023-11-06T16:23:47Z
AUTHORS (7)
ABSTRACT
Introduction The adhesion of flavescence dorée phytoplasma to the midgut epithelium cells their insect vectors is partially mediated by variable membrane protein A (VmpA), an adhesin which shows lectin properties. In order identify receptor for VmpA, we identified Euscelidius variegatus cell proteins interacting with recombinant VmpA-His 6 . Methods E. were mass spectrometry analysis VmpA- complexes formed upon in vitro interaction assays. To assess impact VmpA binding, reduced expression candidate genes on culture dsRNA-mediated RNAi. effect gene knockdown binding was measured capacity bind VmpA-coated fluorescent beads. Results and discussion There 13 possessing potential N-glycosylation sites predicted transmembrane domains selected. decrease unknown leucine-rich repeat (uk1_LRR) correlated decreased beads cells. uk1_LRR more expressed digestive tubes than salivary glands could be implicated early stages infection following phytoplasmas ingestion
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