Extracellular host DNA contributes to pathogenic biofilm formation during periodontitis

• DOI: 10.3389/fcimb.2024.1374817
• Publication Date: 2024-05-08T04:32:46Z
• Authors: Mariana Slobodian...; Svitlana Khlebas; Iryna Mazur; Kateryna Rudnieva; Viktoria Potochilova; Olga Iungin; Olexandr Kamyshnyi; Iryna Kamyshna; Geert Potters; Andrew J. Spiers; Olena Moshynets
• Keywords: DNA, Bacterial; 0301 basic medicine; Microscopy, Confocal; Bacteria; Inflammasomes; Extracellular Polymeric Substance Matrix; Gingiva; DNA; Microbiology; QR1-502; biofilm structure; 03 medical and health sciences; Cellular and Infection Microbiology; FISH; Biofilms; Chronic Periodontitis; Humans; eDNA; Periodontitis; periodontitis; In Situ Hybridization, Fluorescence; subgingival biofilms

Introduction Periodontal diseases are known to be associated with polymicrobial biofilms and inflammasome activation. A deeper understanding of the subgingival cytological (micro) landscape, role extracellular DNA (eDNA) during periodontitis, contribution host immune eDNA persistence, may improve our mechanisms underlaying severe forms periodontitis. Methods In this work, biolfilms developing on biologically neutral polyethylene terephthalate films placed in gingival cavities patients chronic periodontitis were investigated by confocal laser scanning microscopy (CLSM). This allowed examination realistic landscapes visualization polymeric substances (EPS) including amyloids, total proteins, carbohydrates eDNA, as well comparison several single-strain vitro model produced oral pathogens such Klebsiella pneumoniae , Pseudomonas aeruginosa Staphylococcus aureus Streptococcus gordonii S. sanguinis mitis . Fluorescence situ hybridization (FISH) analysis was also used identify derived from eubacteria, streptococci members Bacteroides–Porphyromonas–Prevotella (BPP) group Results Analysis biofilm EPS revealed low levels amyloids high which appears main matrix component. However, bacterial contributed less than a third observed, suggesting that host-derived released neutrophil traps more importance development causing Discussion immunocompetent cells activated at onset therefore major driver persistence pathogenesis.