Minor envelope proteins from GP2a to GP4 contribute to the spread pattern and yield of type 2 PRRSV in MARC-145 cells

• DOI: 10.3389/fcimb.2024.1376725
• Publication Date: 2024-03-25T04:58:35Z
• Authors: Yuan-Zhe Bai; Yue Sun; Yong-Gang Liu; Hong-Liang Zhang; Tong-Qing An; Qian Wang; Zhi-Jun Tian; Xinyuan Qiao; Xue-Hui Cai; Yan-Dong Tang
• Keywords: 0301 basic medicine; Vaccines; Swine; spread pattern; cell-to-cell; Porcine Reproductive and Respiratory Syndrome; yield; Microbiology; Guanidines; QR1-502; Piperazines; Cell Line; 03 medical and health sciences; Cellular and Infection Microbiology; PRRSV; Animals; cell-free; Porcine respiratory and reproductive syndrome virus

In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated live attenuated (LAVs), produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in have a low yield. Here, we used two type 2 (CH-1R HuN4) to identify genes responsible for yield cells. Our findings indicate that viruses different spread patterns, ultimately determine their By replacing viral envelope with reverse genetics system, discovered minor proteins, GP2a GP4, play crucial role determining pattern of The cell-free transmission appears be more efficient than cell-to-cell pattern. Overall, these suggest GP4 contributes PRRSV.