Understanding the vaginal microbiome among women with different genotypes of human papillomavirus infection in remote Andaman islands

• DOI: 10.3389/fcimb.2024.1486166
• Publication Date: 2025-01-15T06:15:50Z
• Authors: Rehnuma Parvez; Santhiya Vijayakumar; Alwin Vins; Sudha Ramaiah; Anand Anbarasu; Lipika Biswas; Nisha Beniwal; Harpreet Kaur; Nagarajan Murugan...
• Keywords: Vaginal infections

Human papillomavirus (HPV) is a viral infection, and its acquisition persistence are significantly influenced by the vaginal microbiota. Understanding comparing microbiome of HPV infected women in Andaman Nicobar Islands crucial. The study involved collecting swabs extracting DNA using QIAamp Minikit. was then subjected to PCR amplification confirm infection. illumina NovaSeq 6000 platform utilized perform sequencing utilizing 2 x 250 paired end chemistry. Taxonomic analysis performed Bacterial abundance plots were generated samples grouped based on demographic parameters, pap test diagnosis, genotypes. To assess diversity, rarefied 49,000 sequence reads per sample, alpha beta diversity metrics calculated. analyzed presence 21 assigned phyla, with Firmicutes, Actinobacteria, Bacteriodetes, Proteobacteria emerging as predominant taxa. At genus level, Lactobacillus Gardnerella dominated across all samples. more abundant HPV-positive (22.40%) compared HPV-negative (10.04%). Symptomatic group had Gardnerella, unclassified Coriobacteriaceae being dominant. In terms bacterial found statistically significant association when comprising individuals aged 30 years those 31 40 years. Most research concluded that exposure can boost vagina healthy women, increasing risk cervical cancer development. Current highlights importance associated high low HPV, various age well symptomatic asymptomatic cases South Andaman.