Dual Function of a in vivo Albumin-Labeling Tracer for Assessment of Blood Perfusion and Vascular Permeability in Peripheral Arterial Disease by PET

• DOI: 10.3389/fcvm.2022.738076
• Publication Date: 2022-02-08T04:46:34Z
• Authors: Zhongchan Sun; Guang Tong; Yuanhui Liu; Hualin Fan; Weibin He; Bo Wang; Shuang Xia; Pengcheng He
• Keywords: Vascular permeability

Peripheral arterial disease (PAD) leads to tissue ischemia in the extremities. Enhanced vascular permeability plays a critical role targeted delivery of drugs for effective therapeutic angiogenesis and resultant blood perfusion recovery. However, optimal tracers evaluating this process PAD patients are lacking. At time, we employed novel vivo albumin-labeling tracer dual function, termed as 18F-NEB, assess well by positron emission tomography (PET).After successful establishment mouse hindlimb (HI) model, static PET imaging was performed 15 min 2 h post injection (p.i.) 18F-NEB at 1, 3, 5, 7, 10 14 days post-surgery respectively. Gradual recovery supply detected scan p.i. collaborated serial Laser Doppler. In addition, highest observed high local uptake consistent with histological examinations. Furthermore, quantitatively evaluated effect endothelial growth factor (VEGF) stimulus on using probe HI which were also confirmed immunohistological results.The application alone can successfully achieve different time points monitor their responses therapy model. The simple labeling approach multipurpose feature suggest great promise theranostic applications treating ischemic disease.