Single-Cell Sequencing of Immune Cells in Human Aortic Dissection Tissue Provides Insights Into Immune Cell Heterogeneity

• DOI: 10.3389/fcvm.2022.791875
• Publication Date: 2022-03-31T09:37:17Z
• Authors: Yifan Liu; Lingwei Zou; Hanfei Tang; Jie Li; Hao Liu; Xiaolang Jiang; Baohong Jiang; Zhihui Dong; Weiguo Fu
• Keywords: Proinflammatory cytokine

Inflammation plays an important role in the progression of sporadic aortic dissection (AD). Immune cells, especially macrophages, infiltrate aorta and secrete inflammatory cytokines matrix metalloproteinases to cause degradation extracellular matrix, thereby contributing pathogenesis AD. However, cellular heterogeneity within these immune cells has not been fully characterized.We used single-cell RNA sequencing profile transcriptomes all AD tissue normal aorta. Using magnetic-activated cell sorting gating on CD45, we obtained a higher resolution identification subsets aorta.We observed significant differences proportion major subpopulations between tissues. Macrophages accounted for percentage aorta, while proportions T B natural killer (NK) were increased Macrophage clusters that expanded tissues originated primarily from circulating monocytes expressed genes encoding proinflammatory molecules involved repair. NK exhibited enhanced cytotoxic properties. A cluster CD4+ had was Th17-like might contribute Cell-cell interaction analysis highlighted communication macrophages which regulated costimulation cells.Our study provides comprehensive characterization dissected with emphasis cells. The information our improves understanding mechanisms formation helps identify additional useful targets early diagnosis or therapy