Differential Gene Expression and Immune Cell Infiltration in Carotid Intraplaque Hemorrhage Identified Using Integrated Bioinformatics Analysis

• DOI: 10.3389/fcvm.2022.818585
• Publication Date: 2022-05-17T07:24:26Z
• Authors: Xiaoshuo Lv; Feng Wang; Mingsheng Sun; Congrui Sun; Xueqiang Fan; Bo Ma; Yuguang Yang; Zhidong Ye; Peng Liu; Jianyan Wen
• Keywords: Pulmonary and Respiratory Medicine; Immunological Mechanisms in Atherosclerosis Development; Immunology; Cancer research; Cardiovascular Medicine; Gene; Pathophysiology and Management of Cerebral Aneurysms; 03 medical and health sciences; Health Sciences; Pathology; Genetics; Diseases of the circulatory (Cardiovascular) system; Biology; Immunology and Microbiology; 0303 health sciences; intraplaque hemorrhage; FOS: Clinical medicine; immune cell infiltration; Life Sciences; bioinformatics; GEO; Diagnosis and Treatment of Carotid Artery Disease; 3. Good health; Plaque Inflammation; Immune system; Neurology; RC666-701; FOS: Biological sciences; Medicine; Gene expression; atherosclerosis

Intraplaque hemorrhage (IPH) is an important feature of unstable plaques and independent risk factor for cardiovascular events. However, the molecular mechanisms contributing to IPH are incompletely characterized. We aimed identify novel biomarkers interventional targets characterize role immune cells in pathogenesis.The microarray dataset GSE163154 which contain non-IPH plaque samples was obtained from Gene Expression Omnibus (GEO). R software adopted identifying differentially expressed genes (DEGs) conducting functional investigation. The hub were carried by protein-protein interaction (PPI) network validated GSE120521 dataset. CIBERSORT deconvolution used determine differential cell infiltration relationship genes. confirmed expression proteins encoded immunohistochemistry western blotting 8 human carotid endarterectomy with without (non-IPH).We detected a total 438 (DEGs), 248 upregulated 190 downregulated. DEGs mainly involved inflammatory related pathways, including neutrophil activation, degranulation, neutrophil-mediated immunity, leukocyte chemotaxis, lysosomes. found through method degree PPI showed that ITGB2 ITGAM might play IPH. Receiver operating characteristic (ROC) results also good performance these two test validation proportions infiltrating differed, especially terms M0 M2 macrophages. Immunohistochemistry analysis levels increased significantly atherosclerotic IPH.ITGB2 key may biological process Our findings advance our understanding underlying pathogenesis provide valuable information directions future research into diagnosis immunotherapy.