Momordica. charantia-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction

• DOI: 10.3389/fcvm.2022.864188
• Publication Date: 2022-04-18T05:14:50Z
• Authors: Wen-Wen Cui; Cong Ye; Kai-Xuan Wang; Xu Yang; Pei-Yan Zhu; Kan Hu; Ting Lan; Lin-Yan Huang; Wan Wang; Bing Gu; Chen Yan; Ping Ma; Su-Hua Qi; Lan Luo
• Keywords: Mitochondrial ROS

Thoracic radiotherapy patients have higher risks of developing radiation-induced heart disease (RIHD). Ionizing radiation generates excessive reactive oxygens species (ROS) causing oxidative stress, while Momordica. charantia and its extract antioxidant activity. Plant-derived extracellular vesicles (EVs) is emerging as novel therapeutic agent. Therefore, we explored the protective effects -derived EVs-like nanovesicles (MCELNs) against RIHD. Using density gradient centrifugation, successfully isolated MCELNs with similar shape, size, markers EVs. Confocal imaging revealed that rat cardiomyocytes H9C2 cells internalized PKH67 labeled time-dependently. In vitro assay identified promoted cell proliferation, suppressed apoptosis, alleviated DNA damage in irradiated (16 Gy, X-ray) cells. Moreover, elevated mitochondria ROS were scavenged by MCELNs, protecting function re-balanced membrane potential. Furthermore, phosphorylation ROS-related proteins was recovered increased ratios p-AKT/AKT p-ERK/ERK treated Last, intraperitoneal administration mitigated myocardial injury fibrosis a thoracic mice model. Our data demonstrated potential The shed light on preventive regime development for radiation-related toxicity.