Identification of immune-related hub genes and miRNA-mRNA pairs involved in immune infiltration in human septic cardiomyopathy by bioinformatics analysis

• DOI: 10.3389/fcvm.2022.971543
• Publication Date: 2022-09-20T04:32:51Z
• Authors: Jingru Li; Guihu Sun; Haocheng Ma; Xinyu Wu; Chaozhong Li; Peng Ding; Si Lu; Yanyan Li; Ping Yang; Chaguo Li; Jun Yang; Yunzhu Peng; Zhaohui Meng; Luqiao Wang
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; miRNA-mRNA regulatory network; RC666-701; septic cardiomyopathy; immune-related gene; biomarker; Diseases of the circulatory (Cardiovascular) system; Cardiovascular Medicine; miRNA; 3. Good health

Septic cardiomyopathy (SCM) is a serious complication caused by sepsis that will further exacerbate the patient's prognosis. However, immune-related genes (IRGs) and their molecular mechanism during septic are largely unknown. Therefore, our study aims to explore hub (IRHGs) miRNA-mRNA pairs with potential biological regulation in SCM means of bioinformatics analysis experimental validation.Firstly, screen differentially expressed mRNAs (DE-mRNAs) from dataset GSE79962, construct PPI network DE-mRNAs. Secondly, were identified overlapped immune cell marker (ICMGs) obtain IRHGs SCM. In addition, receiver operating characteristic (ROC) curve was also performed this process determine disease diagnostic capability IRHGs. Finally, crucial miRNA-IRHG regulatory predicted constructed bioinformatic methods. Real-time quantitative reverse transcription-PCR (qRT-PCR) GSE72380 used validate expression key axis.The results infiltration showed neutrophils, Th17 cells, Tfh central memory cells had more than control group; A total 2 obtained crossing gene ICMGs, validated qRT-PCR. Ultimately, we IRHG SCM: THBS1. The ROC THBS1 area under (AUC) 0.909. miR-222-3p/THBS1 axis constructed.In summary, propose may be IRHG, can serve as biomarker for diagnosis SCM; involved pathogenesis promising candidate therapy.