FISH analysis of numerical chromosomal abnormalities in the sperm of robertsonian translocation der(13; 14)(q10;q10) carriers

• DOI: 10.3389/fgene.2022.1010568
• Publication Date: 2022-09-27T06:42:07Z
• Authors: Saijuan Zhu; Yong Zhu; Feng Zhang; Junping Wu; Ying Chen; Yijuan Sun; Jing Fu; Jiangnan Wu; Min Xiao; Shuo Zhang; Jing Zhou; Caixia Lei; Feng Jiang
• Keywords: Robertsonian translocation

Fluorescence in situ hybridization analysis of numerical chromosomal abnormalities the sperm Robertsonian translocation der (13;14) (q10;q10) carriers has focused on a limited number chromosomes mainly chromosome 13, 18, 21, X, and Y. Here, we aimed to expand all by increasing probes analyzed fluorescence hybridization. The incidence (1–22, Y) was determined from 10 der(13;14)(q10;q10) normozoospermic males fully assess effect translocation-derived segregation during meiosis. Numerical two translocated were frequently detected carriers, with an average frequency 14.55% ± 6.00% for 13 13.27% 4.14% 14. nontranslocated chromosomes, 1.77% 0.62% (range, 1.16%–3.73%), lower than that chromosome. However, cumulative abnormality 22 comparable chromosomes. Significantly increased der(13;14) compared those indicates presence disturbances, being most affected; also affected, but lesser extent due mild interchromosomal effect.