Functional gene polymorphisms and expression alteration of selected microRNAs and the risk of various gastric lesions in Helicobacter pylori-related gastric diseases

• DOI: 10.3389/fgene.2022.1097543
• Publication Date: 2023-01-12T07:01:18Z
• Authors: Qi Liu; Danyan Li; Yunkai Dai; Yunzhan Zhang; Shaoyang Lan; Qi Luo; Jintong Ye; Xu Chen; Peiwu Li; Weijing Chen; Ruliu Li; Ling Hu
• Keywords: 0301 basic medicine; single nucleotide polymorphisms; 03 medical and health sciences; microRNA; Helicobacter pylori; Genetics; interaction; gastric disease; QH426-470; 3. Good health

Background:Helicobacter pylori (Hp) persistent infection is an important pathogenic factor for a series of chronic gastric diseases from gastritis to cancer. Genetic and epigenetic abnormalities microRNAs may play vital role in the pathological evolution mucosa Helicobacter pylori-related (HPGD). This study aimed investigate relationship between miR-146a, miR-196a2, miR-149, miR-499 miR-27a gene single nucleotide polymorphisms (SNPs) their expressions with changes mucosa, further analyze interactions SNPs Hp. Methods: Subjects this included patients diagnosed HPGD healthy controls. MiR-146a rs2910164, miR-196a2 rs11614913, miR-149 rs2292832, rs3746444 rs895819 were genotyped by direct sequencing. Fluorescence quantitative PCR was used detect microRNA expressions. Gene-gene gene-environment evaluated multifactor dimensionality reduction (MDR) method. Results: we found that frequency distribution rs11614913 CT genotype precancerous lesion (GPL) group cancer (GC) significantly higher than normal control (NOR) [adjusted OR = 6.16, 95%CI (1.46-26.03); adjusted 11.83, (1.65-84.72), respectively]. C allele associated increased risk GC 10.14, (2.25-45.77); 3.71, 95%CI(1.46-9.44), The MDR analysis results showed interaction Hp GPL (p 0.004). Meanwhile, expression level NOR, inflammation (CI) early (EPL) groups among Hp-positive subjects. And both EPL group. Also, CI atrophy (GA) groups. Conclusion: affect genetic susceptibility or GC. MiR-196a2 have synergistic effect occurrence development GPL. up-regulation miR-499, caused be mechanism carcinogenesis.