Rice lipid transfer protein, OsLTPL23, controls seed germination by regulating starch-sugar conversion and ABA homeostasis

• DOI: 10.3389/fgene.2023.1111318
• Publication Date: 2023-01-16T05:07:46Z
• Authors: Quanlin Li; Wenxue Zhai; Jiaping Wei; Yanfeng Jia
• Keywords: 2. Zero hunger; 0301 basic medicine; 03 medical and health sciences; OsLTPL23; rice; Genetics; seed germination; abscisic acid (ABA); soluble sugar; QH426-470

Seed germination is vital for ensuring the continuity of life in spermatophyte. High-quality seed usually represents good seedling establishment and plant production. Here, we identified OsLTPL23, a putative rice non-specific lipid transport protein, as an important regulator responsible germination. Subcellular localization analysis confirmed that OsLTPL23 present plasma membrane nucleus. The knockout mutants were generated by CRISPR/Cas9-mediated genome editing, osltpl23 lines significantly germinated slower lower than Nipponbare (NIP). Starch soluble sugar contents measurement showed may have alpha-amylase inhibitor activity, high content be causal agent delayed mutants. Transcript profiles germinating seeds exhibited abscisic acid (ABA)-responsive genes, OsABI3 OsABI5, biosynthesis OsNCED1, OsNCED2, OsNCED3 OsNCED4, are obviously upregulated compared to NIP plants, conversely, ABA metabolism genes OsABA8ox1, OsABA8ox2 OsABA8ox3 stepwise decreased. Further investigations found displays weakened early growth, with elevated gene expresssion catabolism repressive transcription response defence-related OsWRKY45, OsEiN3, OsPR1a, OsPR1b OsNPR1. Integrated indicated exert favorable effect on growth via modulating endogenous homeostasis. Collectively, our study provides insights into roles OsLTPL23-mediated carbohydrate conversion pathway which contributes high-vigor production breeding.