Chromosome-level genome assembly of the largefin longbarbel catfish (Hemibagrus macropterus)

• DOI: 10.3389/fgene.2023.1297119
• Publication Date: 2023-11-01T16:48:26Z
• Authors: Huan Ye; Jiahui Fan; Yanling Hou; Huamei Yue; Rui Ruan; Shuang Li; Chongjiang Hu; Yong Xie; Chuangju Li
• Keywords: Synteny; Sequence assembly

The largefin longbarbel catfish, Hemibagrus macropterus , is an economically important fish species in southwestern China, with males growing faster than females. This study presents a high-quality chromosome-level genome assembly of the generated by integrating Illumina short reads, PacBio HiFi long and Hi-C data. assembled size was 858.5 Mb, contig scaffold N50 5.8 Mb 28.4 respectively. A total 656 contigs were successfully anchored to 30 pseudochromosomes BUSCO score 97.7%, consistent number chromosomes analyzed karyotype. contained 29.5% repeat sequences, predicted 26,613 protein-coding genes, which 25,769 (96.8%) functionally annotated different databases. Evolutionary analysis showed that H. most closely related wyckioides divergence time approximately 16.3 million years. Chromosomal syntenic relationships among Pelteobagrus fulvidraco revealed one-to-one relationship for chromosomes, except break, fission, inversion some chromosomes. first reference will not only provide valuable genetic resource sex determination mechanisms breeding but also contribute comparative analyses chromosome evolution within Siluriformes.