Janus Kinase Inhibitor Baricitinib Modulates Human Innate and Adaptive Immune System

• DOI: 10.3389/fimmu.2018.01510
• Publication Date: 2018-06-28T00:15:12Z
• Authors: Satoshi Kubo; Shingo Nakayamada; Kei Sakata; Yukihiro Kitanaga; Xiaoxue Ma; Seunghyun Lee; Akina Ishii; Kaoru Yamagata; Kazuhisa Nakano; Yoshiya Tanaka
• Keywords: 0301 basic medicine; human immunology; 0303 health sciences; 03 medical and health sciences; tofacitinib; Immunology; baricitinib; Immunologic diseases. Allergy; RC581-607; Janus kinase; signal transducer and activator of transcription; 3. Good health

The purpose of this study was to elucidate the mechanism of action of baricitinib on Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling, which involves in human innate and adaptive immune system. The effects of baricitinib were evaluated using human monocyte-derived dendritic cells (MoDCs), plasmacytoid dendritic cells (pDCs), B cells, and T cells. Baricitinib concentration-dependently suppressed the expression of CD80/CD86 on MoDCs and the production of type-I interferon (IFN) by pDCs. Baricitinib also suppressed the differentiation of human B cells into plasmablasts by B cell receptor and type-I IFN stimuli and inhibited the production of interleukin (IL)-6 from B cells. Human CD4+ T cells proliferated after T cell receptor stimulation with anti-CD3 and anti-CD28 antibody; however, such proliferation was suppressed by baricitinib in a concentration-dependent manner. In addition, baricitinib inhibited Th1 differentiation after IL-12 stimulation and Th17 differentiation by TGF-β1, IL-6, IL-1β, and IL-23 stimulation. Tofacitinib showed similar effects in these experiments. In naive CD4+ T cells, IFN-α and IFN-γ induced phosphorylation of STAT1, which was inhibited by baricitinib and tofacitinib. Furthermore, IL-6-induced phosphorylation of STAT1 and STAT3 was also inhibited by JAK inhibitors. In conclusion, the results indicated that baricitinib suppresses the differentiation of plasmablasts, Th1 and Th17 cells, as well as innate immunity, such as the T cell stimulatory capacity of dendritic cells. Thus, JAK inhibitors can be potentially clinically effective not only in rheumatoid arthritis but other immune-related diseases.