Transcriptome Analysis of Immune Receptor Activation and Energy Metabolism Reduction as the Underlying Mechanisms in Interleukin-6-Induced Skeletal Muscle Atrophy

• DOI: 10.3389/fimmu.2021.730070
• Publication Date: 2021-09-06T06:06:40Z
• Authors: Hualin Sun; Junjie Sun; Ming Li; Lei Qian; Lilei Zhang; Ziwei Huang; Yuntian Shen; Betty Yuen-Kwan Law; Liang Liu; Xiaosong Gu
• Keywords: KEGG; Muscle Atrophy

Background Inflammation may trigger skeletal muscle atrophy induced by cancer cachexia. As a pro-inflammatory factor, interleukin-6 cause atrophy, but the underlying molecular mechanisms have not been explored. Methods In this experimental study, we used adult male ICR mice, weighing 25 ± 2 g, and continuous infusion of into tibialis anterior to construct model (experimental group). A control group received saline infusion. RNA-sequencing was analyze differentially expressed genes in tissue samples after one three days. Gene Ontology Kyoto Encyclopedia Genes Genomes analysis were applied define function these genes, protein-protein interaction performed identify potential transcription factors. Fluorescence microscopy determine fiber cross-sectional area 14 Results Continuous for days caused significant atrophy. found 359 1- 3-day 1748 only samples. Functional showed that both associated with immune receptor activation, whereas sample reduced energy metabolism. The expression multiple oxidative phosphorylation tricarboxylic acid cycle pathways down-regulated. Furthermore, factors identified, their predicted, which indicated STAT3, NF-κB, TP53 MyoG play an important role process interleukin-6-induced Conclusions This study through activation reduction Several downstream IL-6 become new regulators enriches regulation mechanism also provides target targeted therapy