Pandemic Preparedness Against Influenza: DNA Vaccine for Rapid Relief

• DOI: 10.3389/fimmu.2021.747032
• Publication Date: 2021-10-10T13:34:05Z
• Authors: Tor Kristian Ande...; Johanna Bodin; Fredrik Oftung; Bjarne Bogen; Siri Mjaaland; Gunnveig Grødeland
• Keywords: Preparedness; Pandemic; Pandemic influenza; Influenza pandemic

The 2009 “swine flu” pandemic outbreak demonstrated the limiting capacity for egg-based vaccines with respect to global vaccine supply within a timely fashion. New platforms that efficiently can quench influenza emergences are urgently needed. Since 2009, there has been profound development of new platform technologies prophylactic use in population, including DNA vaccines. These particularly well suited responses as format is temperature stable and production process cheap rapid. Here, we show by targeting antigens directly antigen presenting cells (APC), efficacy equals conventional technologies. A single dose naked encoding hemagglutinin (HA) from influenza/A/California/2009 (H1N1), linked moiety directing major histocompatibility complex class II (MHCII) molecules, raised similar humoral immune adjuvanted split virion Pandemrix, widely administered pandemic. Both formats rapidly induced serum antibodies could protect mice already 8 days after immunization, contrast slower kinetics seasonal trivalent inactivated (TIV). Importantly, also elicited cytotoxic T-cell reduced morbidity vaccination, very limited seen immunization Pandemrix TIV. data demonstrate potential vaccine, rapid protective effects without need adjuvant, confirms relevance candidates preparedness.