Proinflammatory Microenvironment During Kingella kingae Infection Modulates Osteoclastogenesis

• DOI: 10.3389/fimmu.2021.757827
• Publication Date: 2021-12-02T10:36:12Z
• Authors: Ayelén Ivana Pesc...; Franco Agustín Sv...; Cinthya Alicia Ma...; Rosa Nicole Freib...; Jorge Quarleri; María Victoria De...
• Keywords: Kingella kingae; Proinflammatory cytokine; Osteolysis

Kingella kingae is an emerging pathogen that causes septic arthritis, osteomyelitis, and bacteremia in children from 6 to 48 months of age. The presence bacteria within or near the bone associated with inflammatory process results osteolysis, but underlying pathogenic mechanisms involved are largely unknown. To determine link between K. loss, we have assessed whether infection per se through genesis a pro-inflammatory microenvironment can promote osteoclastogenesis. For purpose, examined both direct effect immune-mediated mechanism -infected macrophage-induced Our indicate osteoclastogenesis stimulated by directly indirectly fueling potent response drives macrophages undergo functional osteoclasts via TNF-α IL-1β induction. Such osteoclastogenic capability counteracted their outer membrane vesicles (OMV) concentration-dependent manner. In conclusion, this model allowed elucidating interplay OMV modulate exposed macrophages, thus contributing modulation joint damage.