Fish Paralog Proteins RNASEK-a and -b Enhance Type I Interferon Secretion and Promote Apoptosis

• DOI: 10.3389/fimmu.2021.762162
• Publication Date: 2021-11-22T06:32:12Z
• Authors: Zhi-Chao Sun; Zeyin Jiang; Xiaowen Xu; Meifeng Li; Qing Zeng; Ying Zhu; Shanghong Wang; Yuanyuan Li; Xiao-Li Tian; Chengyu Hu
• Keywords: IRF3; IRF7; Grass carp

Type I interferon and apoptosis elicit multifaceted effects on host defense various diseases, such as viral infections cancers. However, the gene/protein network regulating type has not been elucidated completely. In this study, we selected grass carp ( Ctenopharyngodon idella ) an experimental model to investigate modulation of RNASEK secretion apoptosis. We first cloned two paralogs RNASEK-a -b in carp, defined three exons each gene, found length both coding regions is 306 bp with 73.27% protein homology. The sequences are highly conserved across species. Two proteins were mainly localized early late endosomes endoplasmic reticulum. Further, quantitative real-time PCR demonstrated that dsRNA poly I:C reovirus upregulated cells tissues. Overexpression individually induced expression phosphorylation IRF3/IRF7 shown by Western blot immunofluorescent staining, increased Bax / Bcl-2 mRNA ratio, DNA fragmentations, TUNEL-positive cells, proportion Annexin V-positive signals flow cytometry, activated eIF2α, opposite observed when knocked down multiple cell types. Taken together, claim for time fish paralog enhance promote apoptosis, which may be involved respectively. Our study reveals a previously unrecognized role new positive regulator