Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs

• DOI: 10.3389/fimmu.2021.763912
• Publication Date: 2021-11-03T05:34:28Z
• Authors: Eleni Vatzia; Elizabeth R. Allen; Tanuja Manjegowda; Susan Morris; Adam McNee; Veronica Martini; Reshma Kaliath; Marta Ulaszewska; Amy Boyd; Basudev Paudyal; Veronica B. Carr; Tiphany Chrun; Emmanuel Maze; Ronan MacLoughlin; Pauline M. van Di...; Helen E. Everett; Teresa Lambe; Sarah C. Gilbert; Elma Tchilian
• Keywords: Pandemic

There is a critical need to develop superior influenza vaccines that provide broader protection. Influenza are traditionally tested in naive animals, although humans exposed the first years of their lives, but impact prior exposure on vaccine immune responses has not been well studied. Pigs an important natural host for influenza, source pandemic viruses, and excellent model human influenza. Here, we investigated immunogenicity ChAdOx2 viral vectored vaccine, expressing nucleoprotein, matrix protein 1, neuraminidase H1N1pdm09 pre-exposed pigs. We evaluated importance route administration by comparing intranasal, aerosol, intramuscular immunizations. Aerosol delivery boosted local lung T-cell antibody responses, while immunization peripheral blood immunity. These results will inform how best deliver order harness optimal protective