Dynamics of Macrophage, T and B Cell Infiltration Within Pulmonary Granulomas Induced by Mycobacterium tuberculosis in Two Non-Human Primate Models of Aerosol Infection

• DOI: 10.3389/fimmu.2021.776913
• Publication Date: 2022-01-06T05:27:08Z
• Authors: Laura Hunter; Suzie Hingley-Wilson; Graham R. Stewart; Sally A. Sharpe; Francisco Javier ...
• Keywords: CD68

Non-human primate models of Tuberculosis (TB) are one the most commonly used within experimental TB field because they closely mimic whole spectrum disease progression human TB. However, early cellular interactions pulmonary granuloma still not well understood. The use this model allows investigation into cells granulomas which cannot be undertaken in samples. Pulmonary from rhesus and cynomolgus macaques two timepoints post infection were categorised categories 1 – 6 (early to late stage granulomas) immunohistochemistry was identify CD68+ macrophages, CD3+ T CD20+ B cells. Multinucleated giant acid-fast bacilli also quantified. At week four infection, found have more than all but category granulomas. Cynomolgus had a significantly higher percentage twelve abundant 4 5 both species; however, there no significant differences between them. majority compared rhesus. at 12 challenge species. This study has identified basic composition spatial distribution immune over time. data will add knowledge already gained may inform future research on vaccines therapeutics for