Structure of a new glycyrrhiza polysaccharide and its immunomodulatory activity

• DOI: 10.3389/fimmu.2022.1007186
• Publication Date: 2022-09-27T06:29:35Z
• Authors: Yu Wu; Hui Zhou; Kunhua Wei; Tao Zhang; Yanyun Che; Audrey D. Nguyễn; Sakshi Pandita; Xin Wan; Xuejie Cui; Bingxue Zhou; Caiyue Li; Ping Hao; Hongjun Lei; Lin Wang; Xiaonan Yang; Ying Liang; Jiaguo Liu; Yi Wu
• Keywords: Sepharose

A component of licorice polysaccharide (GPS-1) was extracted from licorice, its primary structure identified and characterized for the first time, immunomodulatory activity studied. Crude isolated purified by DEAE sepharose FF ion-exchange column chromatography Chromdex 200 PG gel filtration to obtain a Glycyrrhiza named GPS-1. NMR methylation analysis revealed that GPS-1 is composed homogalacturonan (HG)-type pectin with 4)-D-GalpA-(1 as backbone. This study also examined significant role in regulating immune vitro vivo. As result, promoted secretion IFN-γ IL-4 mice increased proportion CD3+CD4+ CD3+CD8+ T lymphocytes their spleens. Dendritic cells (DCs) treated showed promotion DC maturation, antigen presentation, phagocytic capacity. The results suggest potential immunomodulator stimulates system multiple signaling pathways. Combined our characterization GPS-1, present investigation provides basis future form-function relationship polysaccharides.