Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts

• DOI: 10.3389/fimmu.2022.1019201
• Publication Date: 2022-09-29T16:13:54Z
• Authors: Ives Charlie-Silva; Natália M. Feitosa; Leticia G. Pontes; Bianca H. Fernandes; Rafael H. Nóbrega; Juliana M. M. Gomes; Mariana N. L. Prata; Fausto K. Ferraris; Daniela C. Melo; Gabriel Conde; Letícia F. Rodrigues; Mayumi F. Aracati; José D. Corrêa-Ju...; Wilson G. Manrique; Joshua Superio; Aguinaldo S. Garcez; Katia Conceição; Tania M. Yoshimura; Silvia C. Núñez; Silas F. Eto; Dayanne C. Fernandes; Anderson Z. Freitas; Martha S. Ribeiro; Artem Nedoluzhko; Mônica Lopes-Ferr...; Ricardo C. Borra; Leonardo J. G. Ba...; Andrea C. Perez; Guilheme Malafaia; Thiago M. Cunha; Marco A. A. Belo; Jorge Galindo-Vil...
• Keywords: Carrageenan; Proteome

Regulation of inflammation is a critical process for maintaining physiological homeostasis. The λ-carrageenan (λ-CGN) mucopolysaccharide extracted from the cell wall red algae (Chondrus crispus) capable inducing acute intestinal inflammation, which translated into production phase reactants secreted blood circulation. However, associated mechanisms in vertebrates are not well understood. Here, we investigated crucial factors behind inflammatory milieu λ-CGN-mediated administered at 0, 1.75, and 3.5% (v/w) by i.p. injection peritoneal cavity adult zebrafish (ZF) (Danio rerio). We found that polymorphonuclear leukocytes (neutrophils) lymphocytes infiltrating ZF had short-term persistence. Nevertheless, they generate strong pattern affects systemically enough to produce edema cavity. Consistent with these findings, infiltration, causes notable tissue changes, resulted overexpression several markers protein level. Using reversed-phase high-performance liquid chromatography followed hybrid linear ion-trap mass spectrometry shotgun proteomic approach, identified 2938 plasma proteins among animals injected PBS λ-CGN. First, bioinformatic analysis revealed composition proteome. Interestingly, 72 commonly expressed were recorded treated control groups, but, surprisingly, 2830 novel differentially exclusively λ-CGN-induced group. Furthermore, proteins, compared group 62 got significant (p < 0.05) upregulation λ-CGN-treated group, while remaining ten downregulated. Next, obtained major protein-protein interaction networks between hub clusters λ-CGN induced Moreover, understand molecular underpinnings effects based on unveiled sets, performed structural similarity generated overlapping 3D reconstructions humans during inflammation. Biological pathway pointed activation abundance diverse classical immune reactants, catalytic enzymes, varied supporting response. Together, this information can be used testing finding pharmacological targets treat human diseases.