Differential Transcriptomics Analysis of IPEC-J2 Cells Single or Coinfected With Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus

• DOI: 10.3389/fimmu.2022.844657
• Publication Date: 2022-03-29T11:50:51Z
• Authors: Lina Song; Jing Chen; Pengfei Hao; Yuhang Jiang; Wang Xu; Letian Li; Si Chen; Zihan Gao; Ningyi Jin; Linzhu Ren; Chang Li
• Keywords: Vero cell; Coronavirus

Porcine epidemic diarrhea (PED) and transmissible gastroenteritis (TGE) caused by porcine virus (PEDV) (TGEV) are two highly contagious intestinal diseases in the swine industry worldwide. Notably, coinfection of TGEV PEDV is common piglets with diarrhea-related diseases. In this study, epithelial cells (IPEC-J2) were single or coinfected and/or TGEV, followed comparison differentially expressed genes (DEGs), especially interferon-stimulated (ISGs), between different groups via transcriptomics analysis real-time qPCR. The antiviral activity interferon-induced transmembrane protein 3 (sIFITM3) on infection was also evaluated. results showed that DEGs can be detected infected PEDV, PEDV+TGEV at 12, 24, 48 hpi, number highest 24 hpi. mainly annotated to GO terms binding, immune system process, organelle part, intracellular part. Furthermore, 90 ISGs upregulated during infection, 27 which associated activity, including ISG15, OASL, IFITM1, IFITM3. sIFITM3 significantly inhibit IPEC-J2 monkey Vero cells. Besides, vesicular stomatitis (VSV) replication These indicate has broad-spectrum activity.