Liver immunopathogenesis in fatal cases of dengue in children: detection of viral antigen, cytokine profile and inflammatory mediators

• DOI: 10.3389/fimmu.2023.1215730
• Publication Date: 2023-06-30T17:54:11Z
• Authors: Leandro Junqueira...; Felipe de Andrade...; Lucca de Lima Siq...; Natália Gedeão Sa...; Caio Gonçalves Az...; Jemima Fuentes Ri...; Carlos Alberto Ba...; Rodrigo Basílio-d...; Ronaldo Mohana-Bo...; Jorge José de Car...; Fernando Colonna ...; Marciano Viana Paes; Kíssila Rabelo
• Keywords: Proinflammatory cytokine; CD68

Introduction Dengue virus (DENV), the etiologic agent of dengue fever illness, represents a global public health concern, mainly in tropical and subtropical areas across globe. It is well known that this acute viral disease can progress to severe hemorrhagic stages some individuals, however, immunopathogenic basis development more forms by these patients yet be fully understood. Objective In context, we investigated characterized histopathological features as cytokine profile cell subpopulations present liver tissues from three fatal cases DENV children. Methods Hematoxylin Eosin, Periodic Acid Schiff Picro Sirius Red staining were utilized for analysis. Immunohistochemistry assay was performed characterize inflammatory response expression patterns. Results Vascular dysfunctions such hemorrhage, vascular congestion edema associated with mononuclear infiltrate observedin all cases. Liver exhibited increased presence CD68+ TCD8+ cells high MMP-9, TNF-a, RANTES, VEGFR-2 mediators. Viral replication confirmed detection NS3 protein. Conclusion Taken together, results evidenced key factors may involved alterations children infection.