Comparative analysis of monocyte-derived dendritic cell phenotype and T cell stimulatory function in patients with acute-on-chronic liver failure with different clinical parameters

• DOI: 10.3389/fimmu.2023.1290445
• Publication Date: 2023-12-04T04:33:53Z
• Authors: Zhipeng Wu; Hongbo Shi; Lei Zhang; Honglin Shi; Xingzhong Miao; Liangjuan Chen; Yu Chen; Yingmin Ma
• Keywords: Monocyte; CD86

Background Acute-on-Chronic Liver Failure (ACLF) patients experience systemic inflammation as well immune dysfunction and exhaustion. The phenotype functionality of monocyte-derived dendritic cells in ACLF with different clinical parameters have not been elucidated. Methods This study included 37 cases ACLF, 20 Chronic Hepatitis B (CHB) patients, 12 healthy controls. Demographic laboratory were collected from the enrolled patients. Peripheral blood samples obtained participants. Monocyte-derived induced cultured, followed by co-culturing T Cell surface markers intracellular analyzed using flow cytometry. relationship between these was compared. Results Our found that had lower expression levels HLA-DR, CD86, CD54 on compared to both CHB IL-4, GM-CSF, alcohol promote cells. In higher procalcitonin (PCT), albumin, decreased prothrombin activity deceased associated mononuclear (PBMCs), after removing adherent cells, co-cultured DC. revealed infection low albumin exhibited a proportion cell subsets within PBMCs. Additionally, patients’ showed Ki-67 interferon-gamma (IFN-γ) production. Conclusion exhibit varying states, differences ability stimulate Alcohol can maturation