Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory
0301 basic medicine
0303 health sciences
allergic rhinitis
Stem Cells
Immunology
T-Lymphocytes, Helper-Inducer
RC581-607
Immunoglobulin E
Rhinitis, Allergic
anti-inflammation
Mice
Th1 and Th2 cells
Humans
Animals
Cytokines
human adipose tissue-derived stem cells
Female
Immunologic diseases. Allergy
extracellular vesicles
DOI:
10.3389/fimmu.2023.1302336
Publication Date:
2023-12-08T11:58:01Z
AUTHORS (11)
ABSTRACT
BackgroundHuman adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated the potential therapeutic effects of hADSC-derived extracellular vesicles (hADSC-EVs) on AR.MethodsFemale BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce AR. One day after the last nasal drop, each group received phosphate buffered saline (PBS) or hADSC-EVs treatment. Associated symptoms and biological changes were then assessed.ResultshADSC-EV treatment significantly alleviated nasal symptoms, and reduced inflammatory infiltration. Serum levels of OVA-specific IgE, interleukin (IL)-4 and interferon (IFN)-γ were all significantly reduced. The mRNA levels of IL-4 and IFN-γ in the spleen also changed accordingly. The T helper (Th)1/Th2 cell ratio increased. The treatment efficacy index of hADSC-EV was higher than that of all human-derived MSCs in published reports on MSC treatment of AR. ADSC-EVs exhibited a greater therapeutic index in most measures when compared to our previous treatment involving ADSCs.ConclusionThese results demonstrated that hADSC-EVs could ameliorate the symptoms of AR by modulating cytokine secretion and Th1/Th2 cell balance. hADSC-EVs could potentially be a viable therapeutic strategy for AR. Further animal studies are needed to elucidate the underlying mechanisms and to optimize potential clinical protocols.
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