Extracellular vesicles from adipose stem cells ameliorate allergic rhinitis in mice by immunomodulatory

• DOI: 10.3389/fimmu.2023.1302336
• Publication Date: 2023-12-08T11:58:01Z
• Authors: Wenhan Yang; Zhiyu Pan; Jiacheng Zhang; Lian Wang; Ju lai; Shican Zhou; Zhili Zhang; Kai Fan; Dan Deng; Zhengliang Gao; Shaoqing Yu
• Keywords: 0301 basic medicine; 0303 health sciences; allergic rhinitis; Stem Cells; Immunology; T-Lymphocytes, Helper-Inducer; RC581-607; Immunoglobulin E; Rhinitis, Allergic; anti-inflammation; Mice; Th1 and Th2 cells; Humans; Animals; Cytokines; human adipose tissue-derived stem cells; Female; Immunologic diseases. Allergy; extracellular vesicles

Background Human adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated potential therapeutic hADSC-derived extracellular vesicles (hADSC-EVs) on Methods Female BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce One day after last nasal drop, each group received phosphate buffered saline (PBS) or hADSC-EVs Associated symptoms biological changes then assessed. Results hADSC-EV treatment significantly alleviated symptoms, reduced inflammatory infiltration. Serum levels OVA-specific IgE, interleukin (IL)-4 interferon (IFN)-γ all reduced. The mRNA IL-4 IFN-γ spleen also changed accordingly. T helper (Th)1/Th2 cell ratio increased. efficacy index was higher than that human-derived MSCs published reports MSC ADSC-EVs exhibited a greater most measures when compared our previous involving ADSCs. Conclusion These results demonstrated could ameliorate AR by modulating cytokine secretion Th1/Th2 balance. potentially be viable strategy for Further animal studies are needed elucidate underlying mechanisms optimize clinical protocols.