Quantitative multiplex immunohistochemistry reveals inter-patient lymphovascular and immune heterogeneity in primary cutaneous melanoma

• DOI: 10.3389/fimmu.2024.1328602
• Publication Date: 2024-02-02T12:15:51Z
• Authors: Julia Femel; Cameron Hill; Irineu Illa Bochaca; Jamie L. Booth; Tina G. Asnaashari; Maria M. Steele; Ata S. Moshiri; Hyungrok Do; Judy Zhong; Iman Osman; Sancy A. Leachman; Takahiro Tsujikawa; Kevin P. White; Young H. Chang; Amanda W. Lund
• Keywords: 0303 health sciences; vasculature; Skin Neoplasms; metastasis, immune microenvironment; Immunology; RC581-607; Immunohistochemistry; lymphatic vessels; 03 medical and health sciences; inflammation; melanoma; Tumor Microenvironment; Humans; Immunologic diseases. Allergy; Melanoma; tertiary lymphoid structures; Retrospective Studies; Lymphatic Vessels

IntroductionQuantitative, multiplexed imaging is revealing complex spatial relationships between phenotypically diverse tumor infiltrating leukocyte populations and their prognostic implications. The underlying mechanisms and tissue structures that determine leukocyte distribution within and around tumor nests, however, remain poorly understood. While presumed players in metastatic dissemination, new preclinical data demonstrates that blood and lymphatic vessels (lymphovasculature) also dictate leukocyte trafficking within tumor microenvironments and thereby impact anti-tumor immunity. Here we interrogate these relationships in primary human cutaneous melanoma. MethodsWe established a quantitative, multiplexed imaging platform to simultaneously detect immune infiltrates and tumor-associated vessels in formalin-fixed paraffin embedded patient samples. We performed a discovery, retrospective analysis of 28 treatment-naïve, primary cutaneous melanomas. ResultsHere we find that the lymphvasculature and immune infiltrate is heterogenous across patients in treatment naïve, primary melanoma. We categorized five lymphovascular subtypes that differ by functionality and morphology and mapped their localization in and around primary tumors. Interestingly, the localization of specific vessel subtypes, but not overall vessel density, significantly associated with the presence of lymphoid aggregates, regional progression, and intratumoral T cell infiltrates. DiscussionWe describe a quantitative platform to enable simultaneous lymphovascular and immune infiltrate analysis and map their spatial relationships in primary melanoma. Our data indicate that tumor-associated vessels exist in different states and that their localization may determine potential for metastasis or immune infiltration. This platform will support future efforts to map tumor-associated lymphovascular evolution across stage, assess its prognostic value, and stratify patients for adjuvant therapy.