Microbiota modulates the steroid response to acute immune stress in male mice

• DOI: 10.3389/fimmu.2024.1330094
• Publication Date: 2024-02-01T04:42:07Z
• Authors: Karla Vagnerová; Taťána Gazárková; Martin Vodička; Peter Ergang; Petra Klusoňová; Tomáš Hudcovic; Dagmar Šrůtková; Petra Petr Hermanová; Lucie Nováková; Jiří Pácha
• Keywords: Corticosterone; CYP17A1

Microbiota plays a role in shaping the HPA-axis response to psychological stressors. To examine of microbiota acute immune stressor, we stimulated adaptive system by anti-CD3 antibody injection and investigated expression adrenal steroidogenic enzymes profiling plasma corticosteroids their metabolites specific pathogen-free (SPF) germ-free (GF) mice. Using UHPLC-MS/MS, showed that 4 hours after challenge levels pregnenolone, progesterone, 11-deoxycorticosterone, corticosterone (CORT), 11-dehydroCORT 3α/β-, 5α-, 20α-reduced were increased SPF mice, but GF counterparts, only CORT was increased. Neither stress nor changed mRNA protein steroidogenesis. In contrast, resulted downregulated genes ( Star , Cyp11a1 Hsd3b1 Hsd3b6 ) upregulated 3α-hydroxysteroid oxidoreductase pathway Akr1c21 Dhrs9 testes liver, encoding with 3β-hydroxysteroid dehydrogenase (HSD) Hsd3b2 Hsd3b3 Hsd3b4 Hsd3b5 ), 3α-HSD Akr1c14 20α-HSD Akr1c6 Hsd17b1 Hsd17b2 5α-reductase Srd5a1 activities, except for which upregulated. colon, modulated Hsd11b1 Hsd11b2 stress. These data underline stressor. modulates stress-induced increase C 21 steroids, including those are neuroactive could play alteration HPA axis animals.