MicroRNA-1985 enhances the redox capability of scallop (Patinopecten yessoensis) in response to poly(I:C) stimulation by targeting MNK1

• DOI: 10.3389/fimmu.2025.1556591
• Publication Date: 2025-05-08T04:11:06Z
• Authors: Linghui Yu; Huiqi Deng; Shaohua Liu; Jianpin Xia; Zhenlin Hao; Donghong Yin; Yaoyao Zhan; Yaqing Chang
• Keywords: Patinopecten yessoensis

To clarify the microRNA (miRNA)-target gene axis is involved in response to pathogen-associated molecular pattern (PAMP)-induced oxidative stress shellfish, full-length cDNA of a novel mitogen-activated protein kinase-interacting kinase 1 (MNK1) homolog from scallop Patinopecten yessoensis (PyMNK1) was cloned and characterized. The interaction between miR-1985 PyMNK1 verified, then responses possible function miR-1985, PyMNK1, miR-1985/PyMNK1 poly(I:C) (a classic virus-related PAMP) stimulation P. were explored preliminarily dissected. results indicate: 1) 5354 bp, with high level sequence conservation across mollusks. 2) MiR-1985 bound 3'-UTR negatively regulated expression PyMNK1. 3) may repress relative superoxide dismutase (SOD) by binding its promoter. 4) Both silencing overexpression promoted enzymatic activity SOD. 5) be elevating SOD/catalase axis. summarize, all observations this study indicated that enhance redox capability via miR-1985/PyMNK1/SOD/CAT cascade thereby alleviate PAMP-induced stress.