Gene silencing in adult Popillia japonica through feeding of double‐stranded RNA (dsRNA) complexed with branched amphiphilic peptide capsules (BAPCs)

• DOI: 10.3389/finsc.2023.1151789
• Publication Date: 2023-05-12T04:36:20Z
• Authors: Elijah Carroll; Nitish Kunte; Erin McGraw; Sujan Gautam; Ryan Range; Jose A. Noveron-N...; David W. Held; L. Adriana Avila
• Keywords: RNA Silencing; Intestinal epithelium

Gene silencing by feeding double-stranded (dsRNA) holds promise as a novel pest management strategy. Nonetheless, degradation of dsRNA in the environment and within insect gut, well inefficient systemic delivery are major limitations to applying this Branched amphiphilic peptide capsules (BAPCs) complexed with have been used successfully target genes outside inside gut epithelium upon ingestion. This suggests that BAPCs can protect from shuttle it across epithelium. In study, our objectives were 1) Determine whether on BAPC-dsRNA complexes targeting putative peritrophin gene P. japonica would result suppression synthesis, 2) gain insight into cellular uptake mechanisms transport larval midgut japonica. Our results suggest readily taken up epithelium, treatment tissue endocytosis inhibitors effectively suppresses intracellular transport. Further, assessment expression BAPC- fed beetles demonstrated significant downregulation mRNA levels relative control and/or alone. increase efficacy knockdown alone adults. To knowledge, is first report nanoparticle-mediated through .