Mesopelagic Fish Protein Hydrolysates and Extracts: A Source of Novel Anti-Hypertensive and Anti-Diabetic Peptides

• DOI: 10.3389/fmars.2021.719608
• Publication Date: 2021-09-04T10:34:20Z
• Authors: Azza S. Naik; R. D. Whitaker; S. Albrektsen; Runar G. Solstad; Lars Thoresen; Maria Hayes
• Keywords: Mesopelagic zone

The abundance of fish and zooplankton (1 × 10 9 tons to 7 tons) in the mesopelagic zone ocean is a source novel raw materials that provides opportunities for sustainable new product development. peculiar conditions light pressure this ecological position specific organisms marine food chain results diversity their bio-composition. Mesopelagic are an underutilized resource, rich proteins omega-3 oils, present develop feed, functional ingredients products. However, there also need ensure resource not overfished processed optimize catch line with sustainability goals. There therefore establish bioprocessing technologies yield value added products from species. In study, various protein extracts Maurolicus muelleri (M) Meganyctiphanes norvegica (Northern Krill) (K) combinations these species (C) were generated using hydrolysis methods. Protein Hydrolysates four different enzymes including Alcalase, endocut-01, endogenous M/K FoodPro PNL. characterized assessed ability inhibit important diseases associated metabolic syndrome. Angiotensin-1-converting enzyme (ACE-1; EC. 3.4.15.1) blood regulation, Acetylcholinesterase (AChE; EC 3.1.1.7) maintenance nervous system, Dipeptidyl peptidase IV (DPP-IV; 3.4.14.5) linked development type-2-diabetes, was determined. separate process, same transformed into fishmeal, Hydrolysates, fish-silage, aqueous (AQ) screened bioactivities bioassays. contained greater than 60% (dry weight basis) when analyzed DUMAS method. A hydrolysate M. PNL (M1) inhibited ACE-1 by 89.56% assayed at concentration 1 mg/ml compared positive control Captopril © . Aqueous extract two (AQ2) 95.28% control. Sample M1 DPP-IV 100% one (AQ1) 90.08% Sitagliptin used as All samples did significantly AChE–fraction C3 (combined hydrolzate 3: Krill ) AChE 27.48% only. Based on M1, C3, AQ1 selected further characterization IC 50 values each determined relation inhibition well amino acid composition. Glutamate aspartate most abundant acids samples. <0.2 distinct terminal identified three fractions. study shows targeting processing have potential generate use prevention type-2-diabetes hypertension.