Secretory and transcriptomic responses of mantle cells to low pH in the Pacific oyster (Crassostrea gigas)

• DOI: 10.3389/fmars.2023.1156831
• Publication Date: 2023-04-11T04:50:37Z
• Authors: Nicolás Zúñiga-Soto; Ingrid Pinto-Borg...; Claudio Quevedo; Felipe Aguilera
• Keywords: Ocean Acidification; Marine invertebrates

Since the Industrial Revolution, concentration of atmospheric carbon dioxide (CO 2 ) due to anthropogenic activities has increased at unprecedented rates. One-third CO emissions are dissolved in oceans affecting chemical equilibrium seawater, which turn leads a decrease pH and carbonate ion 3 2- concentration, phenomenon known as ocean acidification (OA). This disequilibrium can be detrimental marine organisms (e.g., mollusks) that fabricate mineralized structures based on calcium (CaCO ). Most studies effect reduced seawater have been conducted early developmental stages shell-building invertebrates, given less attention how adult individuals face OA stress. Here, we evaluate histological, secretory, transcriptional changes mantle oysters ( Crassostrea gigas exposure ambient (8.0 ± 0.2) (7.6 during 20 days. histological observations did not show differences terms cell morphology. However, Alcian Blue/PAS staining revealed significant number Blue positive cells edge, suggesting secretory activity this morphogenetic zone. Transcriptomic analysis 172 differentially expressed genes (DEGs) between tissues from kept normal conditions. Almost 18% DEGs encode secreted proteins likely contributing shell fabrication patterning. 17 31 encoding correspond oyster-specific genes, highlighting fact molluscan formation is underpinned by rapidly evolving secretome. The GO showed they involved cellular response stimulus, stress, protein binding, these biological processes molecular functions altered OA. study demonstrates histology gene expression profiling advance our understanding mechanisms underlying oyster tolerance low