Composition and Diversity of the Ocular Surface Microbiota in Patients With Blepharitis in Northwestern China

• DOI: 10.3389/fmed.2021.768849
• Publication Date: 2021-12-07T10:38:06Z
• Authors: Changhao Wang; Xiuhong Dou; Jian Li; Jie Wu; Yan Cheng; Na An
• Keywords: Blepharitis; Akkermansia

Purpose: To investigate the composition and diversity of microbiota on ocular surface patients with blepharitis in northwestern China via 16S rDNA amplicon sequencing. Methods: Thirty-seven divided into groups anterior, posterior mixed twenty healthy controls from were enrolled study. Samples collected eyelid margin conjunctival sac each participant. The V3-V4 region bacterial sample was amplified sequenced Illumina HiSeq 2500 sequencing platform, differences taxonomy among different compared. Results: similar to that subjects, but there relative abundance bacterium. At phylum level, abundances Actinobacteria, Cyanobacteria, Verrucomicrobia, Acidobacteria, Chloroflexi, Atribacteria significantly higher group than control group, while Firmicutes lower (p < 0.05, Mann-Whitney U). genus Lactobacillus, Ralstonia, Bacteroides, Akkermansia, Bifidobacterium, Escherichia-Shigella, Faecalibacterium, Brevibacterium Bacillus, Staphylococcus, Streptococcus, Acinetobacter anterior blepharitis. Lactobacillus Bifidobacterium are biomarkers blepharitis, Ralstonia is a biomarker There no significant difference between or without Conclusion: varied study groups, according analysis. reason might be due participants being environments having lifestyles. Bacteroides may play important roles pathogenesis