Stratification of Diversity and Activity of Methanogenic and Methanotrophic Microorganisms in a Nitrogen-Fertilized Italian Paddy Soil

• DOI: 10.3389/fmicb.2017.02127
• Publication Date: 2017-11-12T23:26:57Z
• Authors: Annika Vaksmaa; Theo A. van Alen; Katharina F. Ettwig; Elisabetta Lupotto; Giampiero Valè; Mike S. M. Jetten; Claudia Lüke
• Keywords: Thaumarchaeota; Paddy field; Acidobacteria; Euryarchaeota; Methanogen

Paddy fields are important ecosystems, as rice is the primary food source for about half of world's population. impacted by nitrogen fertilization and a major anthropogenic methane. Microbial diversity methane metabolism were investigated in upper 60cm paddy soil qPCR, 16S rRNA gene amplicon sequencing anoxic 13C-CH4 turnover with suite electron acceptors. The bacterial community consisted mainly Acidobacteria, Chloroflexi, Proteobacteria, Planctomycetes Actinobacteria. Among archaea, Euryarchaeota Bathyarchaeota dominated over Thaumarchaeota 30cm soil. constituted up to 45% total archaeal reads top 5cm. In methanogenic community, Methanosaeta generally more abundant than versatile Methanosarcina. measured maximum production rate was 444 nmol gdwh-1, rates nitrate-, nitrite- iron-dependent anaerobic oxidation (AOM) 57 nmol, 55 56 respectively, at different depths. qPCR revealed higher abundance 'Candidatus Methanoperedens nitroreducens' methanotrophic NC10 phylum bacteria all depths, except 60cm. These results demonstrate that there substantial potential fertilized fields, archaea contributor.