Clostridium cellulovorans Proteomic Responses to Butanol Stress

• DOI: 10.3389/fmicb.2021.674639
• Publication Date: 2021-07-21T16:38:03Z
• Authors: Paolo Costa; Giulia Usai; Angela Re; Marcello Manfredi; Giuseppe Mannino; Cinzia Margherita...; Enrica Pessione; Roberto Mazzoli
• Keywords: Proteome

Combination of butanol-hyperproducing and hypertolerant phenotypes is essential for developing microbial strains suitable industrial production bio-butanol, one the most promising liquid biofuels. Clostridium cellulovorans among with highest potential direct n -butanol from lignocellulosic wastes, a process that would significantly reduce cost bio-butanol. However, butanol exhibits higher toxicity compared to ethanol C. tolerance this solvent low. In present investigation, comparative gel-free proteomics was used study response challenge understand mechanisms activated in condition. Sequential Window Acquisition all Theoretical fragment ion spectra Mass Spectrometry (SWATH-MS) analysis allowed identification quantification differentially expressed soluble proteins. The data are available via ProteomeXchange identifier PXD024183. important concerned modulation protein biosynthesis, folding degradation. Coherent previous studies on other bacteria, several heat shock proteins (HSPs), involved quality control, were up-regulated such as chaperones GroES (Cpn10), Hsp90, DnaJ. Globally, our indicate biosynthesis reduced, likely not overload HSPs. Several additional metabolic adaptations triggered by exposure up-regulation V- F-type ATPases (involved ATP synthesis/generation proton motive force), enzymes amino acid (e.g., arginine, lysine, methionine, branched chain acids) cell envelope re-arrangement products Clocel_4136, Clocel_4137, Clocel_4144, Clocel_4162 Clocel_4352, saturated fatty redistribution carbon flux through fermentative pathways (acetate formate yields increased decreased, respectively). Based these experimental findings, gene targets engineering strategies aimed at improving suggested. This includes overexpression HSPs GroES, DnaJ, ClpC), RNA chaperone Hfq, number genes whose function currently unknown.