Blood Meals With Active and Heat-Inactivated Serum Modifies the Gene Expression and Microbiome of Aedes albopictus

• DOI: 10.3389/fmicb.2021.724345
• Publication Date: 2021-09-09T11:14:20Z
• Authors: Arley Calle-Tobón; Andres F. Holguin...; Celois Moore; Meagan Rippee-Brooks; Paula Rozo-Lopez; Jania Harrod; Soheila Fatehi; Guillermo L. Rua-...; Yoonseong Park; Berlin Londoño-Re...
• Keywords: Aedes albopictus; Human virome; Blood meal

The Asian “tiger mosquito” Aedes albopictus is currently the most widely distributed disease-transmitting mosquito in world. Its geographical expansion has also allowed of multiple arboviruses like dengue, Zika, and chikungunya, to higher latitudes. Due enormous risk global public health caused by mosquitoes species vectors human disease, challenges slowing their expansion, it necessary develop new environmentally friendly vector control strategies. Among these, host-associated microbiome-based strategies have emerged as promising options. In this study, we performed an RNA-seq analysis on dissected abdomens Ae. females from Manhattan, KS, United States fed with sugar blood containing either normal or heat-inactivated serum, evaluate effect heat inactivation gene expression, bacteriome transcripts RNA virome species. Our results showed at least 600 genes modified expression profile when were vs. heat-inactivated-containing blood. These mainly involved immunity, oxidative stress, lipid metabolism, oogenesis. Also, observed changes increase Actinobacteria, Rhodospirillaceae, Anaplasmataceae 6 h post-feeding. We found that feeding seems particularly influence Wolbachia demonstrated a significant bacteria serum. However, no differences core population. suggest further complement proteins serum may profound microbiome which could interpretation pathogen-host interaction findings using type reagents specially measuring competence.