Emerging Severe Acute Respiratory Syndrome Coronavirus 2 Mutation Hotspots Associated With Clinical Outcomes and Transmission

• DOI: 10.3389/fmicb.2021.753823
• Publication Date: 2021-10-19T18:59:33Z
• Authors: Xianwu Pang; Pu Li; Lifeng Zhang; Lusheng Que; Min Dong; Bo Xie; Qihui Wang; Yinfeng Wei; Xing Xie; Lanxiang Li; Chunyue Yin; Liuchun Wei; Kexin Huang; Yiming Hua; Qingniao Zhou; Yingfang Li; Lei Yu; Weidong Li; Zengnan Mo; Maosheng Zhang; Jing Leng; Yanling Hu
• Keywords: Coronavirus; 2019-20 coronavirus outbreak

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing disease 2019 (COVID-19) pandemic. Understanding influence mutations in SARS-CoV-2 gene on clinical outcomes critical for treatment and prevention. Here, we analyzed all high-coverage complete sequences from GISAID database January 1, 2020, to 2021, mine mutation hotspots associated with outcome developed a model predict different epidemic strains. Exploring based RNA-dependent RNA polymerase (RdRp) RNA-editing enzyme, was more likely occur severe mild cases than asymptomatic cases, especially A > G, C T, G mutations. The were mainly open reading frame 1ab (ORF1ab) N genes; R6997P V30L occurred together correlated high prevalence. D614G, Q57H, S194L Interestingly, single-nucleotide variant (SNV) frequency higher percentage nt14408 RdRp cases. expression ADAR APOBEC outcome. has shown that increased over time, while there symptomatic Alpha, Beta, Gamma. These findings suggest genome may have direct association Our result are helpful prevalence strains further study mechanism causing disease.