Expanding Characterized Diversity and the Pool of Complete Genome Sequences of Methylococcus Species, the Bacteria of High Environmental and Biotechnological Relevance

• DOI: 10.3389/fmicb.2021.756830
• Publication Date: 2021-10-07T13:05:41Z
• Authors: Igor Y. Oshkin; Olga V. Danilova; Sergey Y. But; Kirill K. Miroshn...; Ruslan Z. Suleimanov; Svetlana E. Belova; Ekaterina N. Tikh...; Nikolai N. Kuznetsov; Valentina N. Khme...; Nikolai V. Pimenov; Svetlana N. Dedysh
• Keywords: Alphaproteobacteria

The bacterial genus Methylococcus , which comprises aerobic thermotolerant methanotrophic cocci, was described half-a-century ago. Over the years, a member of this genus, capsulatus Bath, has become major model organism to study genomic and metabolic basis obligate methanotrophy. High biotechnological potential fast-growing species, mainly as promising source feed protein, also been recognized. Despite big research attention, currently cultured diversity is represented by members two M. geothermalis while finished genome sequences are available only for strains these methanotrophs. This extends pool phenotypically characterized with good-quality contributing four novel isolates bacteria from activated sludge, landfill cover soil, freshwater sediments. determined sizes varied between 3.2 4.0Mb. As revealed phylogenomic analysis, IO1, BH, KN2 affiliate strain Mc7 may potentially represent species. Highest temperature optima (45–50°C) highest growth rates in bioreactor cultures (up 0.3h −1 ) were recorded obtained sludge. comparative analysis all complete genomes species 4,485 gene clusters. Of these, pan-genome core comprised 2,331 genes (on average 51.9% each genome), accessory containing 846 1,308 shell cloud, respectively. Independently isolation source, displayed surprisingly high synteny striking similarity content. Strain soil differed other content mobile genetic elements number genome-encoded features missing such sucrose biosynthesis ability scavenge phosphorus sulfur environment.