Proteomics Analysis of Exosomes From Patients With Active Tuberculosis Reveals Infection Profiles and Potential Biomarkers

• DOI: 10.3389/fmicb.2021.800807
• Publication Date: 2022-01-06T07:42:09Z
• Authors: Min Zhang; Yiping Xie; Shasha Li; Xiaojian Ye; Yibiao Jiang; Lijun Tang; Jianjun Wang
• Keywords: Exosome; Proteome

Although mycobacterial proteins in exosomes from peripheral serum of patients with tuberculosis (TB) have been identified, other exact compositions remain unknown. In the present study, a comprehensive proteomics analysis derived active TB (ATB) was performed. Exosomes ATB were characterized using nanoparticle tracking (NTA), transmission electron microscopy (TEM), and western blotting analysis. Then identified protein components quantified by label-free determined via bioinformatics A total 123 differential analyzed Gene Ontology (GO) Among these heat shock protein70 (HSP70), CD81, major histocompatibility complex-I (MHC-I ) tumor susceptibility gene101 (TSG101) normal individuals confirmed blotting. addition, among exosomal lipopolysaccharide binding (LBP) increased significantly, but CD36 MHC-I decreased significantly exosomes. Meanwhile, down-expressed blood mononuclear cells (PBMCs) ATB, interestingly down-regulated up-expressed PBMCs validated ELISA flow cytometry. up-regulated M. H37Ra infection macrophages suppressed infected detected This study provided description exosome proteome revealed certain potential biomarkers associated infection.