Heat Stress Affects Faecal Microbial and Metabolic Alterations of Rabbits

Eubacterium Lachnospiraceae Metabolic pathway
DOI: 10.3389/fmicb.2021.817615 Publication Date: 2022-02-28T15:15:44Z
ABSTRACT
Heat stress can impair the rabbit immune system, induce oxidative stress, and cause many complications. These diseases are characterized by metabolic disorders, but underlying mechanism is unknown. As a result, current research determines effects of HS on intestinal microorganisms in rabbits pathway disorders caused HS. Twelve were randomly assigned to one two groups: CON (22-24°C) (30°C-32°C). Both groups treated for 15 days. Blood fecal samples collected day 15. Serum oxidation indices determined using commercial ELISA kit, microbiome feces was studied 16S rRNA gene sequencing. Non-targeted metabolomics analyzed ultra-high-performance liquid chromatography-mass spectrometry (UHPC MS/MS). The findings revealed that significantly increased IgG T-AOC levels serum, whereas it decreased TNF-α IL-10. NMDS analysis substantial difference bacterial community composition between groups. At phylum level, abundance Firmicutes, Protobacteria, Verrucomicrobiota higher group, Bacteriodota reduced group. V9D2013 Haloplasma, Comamonas, Clostridium sensu stricto 1, Ruminiclostridium, Syntrophus Lutispora, at genus level Syntrophorhabdus, Paeniclostridium, 6, Candidatus Caldatribacterium, Spirochaeta Synergistaceae, Syner-01, [Eubacterium] xylanophilum Cellulosilyticum, ADurb.Bin120, Devosia upregulated metabolism group considerably compared with according principal component (PCA) least-squares discriminant (PLS-DA). concentrations 4-pyridoxic acid, kynurenine, 20-OH-leukotriene B4, dopamine concentration pyridoxal. In gut, these compounds primarily impact pathways vitamin B6, tryptophan, neutrophil activation, prolactin. 4-Pyridoxic pyridoxal, essential inflammatory response markers stress.
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