Altered gut fungi in systemic lupus erythematosus – A pilot study

• DOI: 10.3389/fmicb.2022.1031079
• Publication Date: 2022-12-05T06:46:01Z
• Authors: Bao-Zhu Li; Hua Wang; Xian-Bao Li; Qian-Ru Zhang; Rong-Gui Huang; Hong Wu; Yi-Yu Wang; Kai-Di Li; Xiu-Jie Chu; Nv-Wei Cao; Hao-Yue Zhou; Xin-Yu Fang; Rui-Xue Leng; Yin-Guang Fan; Jin-Hui Tao; Zong-Wen Shuai; Dong-Qing Ye
• Keywords: rheumatoid arthritis; ITS sequencing; 0303 health sciences; 03 medical and health sciences; systemic lupus erythematosus; gut fungi; autoimmune diseases; Microbiology; undifferentiated connective tissue diseases; QR1-502; 3. Good health

Objective Gut fungi, as symbiosis with the human gastrointestinal tract, may regulate physiology via multiple interactions host cells. The plausible role of fungi in systemic lupus erythematosus (SLE) is far from clear and need to be explored. Methods A total 64 subjects were recruited, including SLE, rheumatoid arthritis (RA), undifferentiated connective tissue diseases (UCTDs) patients healthy controls (HCs). Fecal samples collected. bacteria detected by ITS sequencing 16S rRNA gene sequencing, respectively. Alpha beta diversities microbiota analyzed. Linear discriminant analysis effect size was performed identify abundance different groups. correlation network between bacterial fungal analyzed based on Spearman correlation. Results diversity community composition exhibited significant shifts SLE compared UCTDs, RA HCs. Compared HCs, alpha decreased patients. According principal coordinates results, constitution RA, UCTDs HCs distinct differences a separation microbiota. There dysbiosis compositions patients, Pezizales, Cantharellales Pseudaleuria enriched UCTDs. complex relationship microbiota, especially Candida which related variety bacteria. Conclusion This study presents pilot identifies several gut patterns taxa among Furthermore, bacterial-fungal association altered