Extracellular membrane vesicles from Limosilactobacillus reuteri strengthen the intestinal epithelial integrity, modulate cytokine responses and antagonize activation of TRPV1

• DOI: 10.3389/fmicb.2022.1032202
• Publication Date: 2022-11-17T04:54:24Z
• Authors: Yanhong Pang; Ludwig Ermann Lun...; Manuel Mata Forsberg; David Ahl; Helena Bysell; Anton Pallin; Eva Sverremark-Ek...; Roger Karlsson; Hans Jonsson; Stefan Roos
• Keywords: Lactobacillus reuteri

Bacterial extracellular membrane vesicles (MV) are potent mediators of microbe-host signals, and they not only important in host-pathogen interactions but also for the between mutualistic bacteria their hosts. Studies MV derived from probiotics could enhance understanding these universal signal entities, here we have studied Limosilactobacillus reuteri DSM 17938 BG-R46. The production increased with cultivation time after oxygen stress. Mass spectrometry-based proteomics analyses revealed that carried a large number bacterial cell surface proteins, several predicted to be involved host-bacteria interactions. A 5'-nucleotidase, which catalyze conversion AMP into molecule adenosine, was one analysis enzymatic activity showed L. BG-R46 exhibited highest activity. We detected TLR2 activator lipoteichoic acid on MV. In models host interactions, first observed were internalized by Caco-2/HT29-MTX epithelial cells, dose-dependent manner decreased leakage caused enterotoxigenic Escherichia coli up 65%. Furthermore, upregulated IL-1β IL-6 peripheral blood mononuclear cells (PBMC), dampened IFN-γ TNF-α responses PBMC challenged Staphylococcus aureus. Finally, strains an antagonistic effect pain receptor transient potential vanilloid 1 model primary dorsal root ganglion rats. summary, shown mobile nanometer scale reproduce biological effects parameters selection strain impact This potentially provide key information development innovative more efficient probiotic products.