Biofilm and pathogenic factor analysis of Gardnerella vaginalis associated with bacterial vaginosis in Northeast China

• DOI: 10.3389/fmicb.2022.1033040
• Publication Date: 2022-12-22T12:00:46Z
• Authors: Xiaolu Ma; Xiaoxi Wang; Shengna Ye; Jinnan Liu; Hong Yuan; Nan Wang
• Keywords: Gardnerella vaginalis; Antibiotic sensitivity

Introduction Gardnerella vaginalis is a major pathogen responsible for bacterial vaginosis (BV). However, the recurrence of infection and antibiotic resistance biofilms remain significant challenges treatment BV. In this study, we aimed to analyze pathogenic factors drug sensitivity associated with clinical BV in Northeast China. Methods Subgroups were identified by clade-specific polymerase chain reaction (PCR). Biofilm formation was measured crystal violet staining, confocal laser scanning microscopy (CLSM) electron (SEM). The inhibition eradication biofilm XTT broth recovery-based methods. Results Of 24 samples G. , 11 American Type Culture Collection (ATCC) 14018 formed biofilms; remainder did not. positive rates detection sialidase A vly genes 100% 79.2%, respectively. Moreover, 21 (87.5%) showed metronidazole 16 (66.7%) presented towards clindamycin. MIC 80 (BMIC ) ATCC14018 μg/ml while that clindamycin 0.125 μg/ml. minimum concentration (MBEC) > 256 2 Discussion Our results revealed more resistant than neither nor are able effectively eradicate vaginal biofilms. Thus, role antibiotics requires further investigation.