Enhancing the ethanol production by exploiting a novel metagenomic-derived bifunctional xylanase/β-glucosidase enzyme with improved β-glucosidase activity by a nanocellulose carrier

• DOI: 10.3389/fmicb.2022.1056364
• Publication Date: 2023-01-04T11:25:52Z
• Authors: Shohreh Ariaeenejad; Elaheh Motamedi; Kaveh Kavousi; Rezvaneh Ghasemit...; Razieh Goudarzi; Ghasem Hosseini S...; Behrouz Zolfaghari; Swapnoneel Roy
• Keywords: Phosphofructokinase 2; Beta-glucosidase

Some enzymes can catalyze more than one chemical conversion for which they are physiologically specialized. This secondary function, is called underground, promiscuous, metabolism, or cross activity, recognized as a valuable feature and has received much attention developing new catalytic functions in industrial applications. In this study, novel bifunctional xylanase/β-glucosidase metagenomic-derived enzyme, PersiBGLXyn1, with underground β-glucosidase activity was mined by in-silico screening. Then, the corresponding gene cloned, expressed purified. The PersiBGLXyn1 improved degradation efficiency of organic solvent pretreated coffee residue waste (CRW), subsequently production bioethanol during separate enzymatic hydrolysis fermentation (SHF) process. After characterization, enzyme immobilized on nanocellulose (NC) carrier generated from sugar beet pulp (SBP), remarkably up to four-fold at 80°C two-fold pH 4.0 compared free one. demonstrated 12 13-fold rise half-life 70 its activity. amount reducing produced saccharification CRW also enhanced 12.97 g/l 19.69 immobilization enzyme. Bioethanol 29.31 after 72 h fermentation, while showed 51.47 titre. Overall, study presented cost-effective metagenomic approach identify It efficacy activities promising alternative fermentable sugars subsequent value-added products.